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Published on: May 4, 2012
A versatile monoclonal antibody specific to human SERPINB5
Sonia S Y Teoh1, Hong Wang, Gail P Risbridger
1Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia.
Hybridoma (2005)
|October 27, 2012
Summary
Researchers developed a new monoclonal antibody, 16F7, that recognizes human maspin (SERPINB5) in all forms. This versatile tool will aid future studies on maspin's tumor suppressor functions.
Area of Science:
- Biochemistry
- Immunology
- Oncology
Background:
- Maspin (SERPINB5) is a serine protease inhibitor belonging to Clade B.
- Maspin is hypothesized to function as a tumor suppressor, but its precise molecular mechanisms require further investigation.
Purpose of the Study:
- To characterize a novel mouse monoclonal antibody, designated 16F7, targeting human maspin.
- To assess the antibody's utility for studying maspin's biological functions.
Main Methods:
- Development and characterization of a mouse monoclonal antibody against human maspin.
- Comparative analysis of antibody binding to maspin in native and denatured states.
- Evaluation against other commercially available maspin antibodies.
Main Results:
- The 16F7 antibody successfully recognizes human maspin.
- 16F7 demonstrates reactivity with maspin in both native and denatured conformations.
- This antibody exhibits superior recognition compared to several other commercial antibodies.
Conclusions:
- The 16F7 monoclonal antibody is a highly specific and versatile tool for maspin detection.
- This antibody will facilitate future research into the molecular roles and tumor suppressor activities of maspin.
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