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A versatile monoclonal antibody specific to human SERPINB5

Sonia S Y Teoh1, Hong Wang, Gail P Risbridger

  • 1Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia.

Hybridoma (2005)
|October 27, 2012
PubMed

Insights

Researchers developed a new monoclonal antibody, 16F7, that recognizes human maspin (SERPINB5) in all forms. This versatile tool will aid future studies on maspin's tumor suppressor functions.

Area of Science:

  • Biochemistry
  • Immunology
  • Oncology

Background:

  • Maspin (SERPINB5) is a serine protease inhibitor belonging to Clade B.
  • Maspin is hypothesized to function as a tumor suppressor, but its precise molecular mechanisms require further investigation.

Purpose of the Study:

  • To characterize a novel mouse monoclonal antibody, designated 16F7, targeting human maspin.
  • To assess the antibody's utility for studying maspin's biological functions.

Main Methods:

  • Development and characterization of a mouse monoclonal antibody against human maspin.
  • Comparative analysis of antibody binding to maspin in native and denatured states.
  • Evaluation against other commercially available maspin antibodies.

Main Results:

  • The 16F7 antibody successfully recognizes human maspin.
  • 16F7 demonstrates reactivity with maspin in both native and denatured conformations.
  • This antibody exhibits superior recognition compared to several other commercial antibodies.

Conclusions:

  • The 16F7 monoclonal antibody is a highly specific and versatile tool for maspin detection.
  • This antibody will facilitate future research into the molecular roles and tumor suppressor activities of maspin.

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