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Published on: May 4, 2012
A mouse monoclonal antibody specific for calreticulin
Gongze Wang1, Jianlin Yang, Chaoqi Liu
1Institute of Molecular Biology, Medical College of Three Gorges University, YiChang, HuBei, China.
Hybridoma (2005)
|October 27, 2012
Summary
Researchers developed a new monoclonal antibody (MAb) that specifically recognizes calreticulin (CRT). This antibody is a valuable tool for studying CRT
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Calreticulin (CRT) is a versatile heat shock protein (HSP) primarily located in the endoplasmic reticulum (ER) lumen.
- Understanding CRT's functions requires specific tools for its detection and study.
Purpose of the Study:
- To develop and characterize a novel monoclonal antibody (MAb) for mouse calreticulin (CRT).
- To validate the MAb's specificity and reactivity for research applications.
Main Methods:
- Expression and purification of a specific region of mouse CRT (dCRT) in E. coli.
- Immunization of BALB/c mice with dCRT to generate hybridoma cell lines.
- Characterization of the MAb using ELISA, Western blot, immunofluorescence, and flow cytometry.
Main Results:
- A stable hybridoma cell line producing a CRT-specific MAb was successfully established.
- The MAb demonstrated high specificity for CRT via ELISA and Western blot.
- The MAb showed excellent reactivity with ecto-CRT in cells induced to undergo apoptosis.
Conclusions:
- A novel MAb targeting mouse calreticulin has been developed and validated.
- This MAb is a promising tool for investigating CRT's diverse functions, particularly its role in apoptosis.
- Further research utilizing this MAb will enhance our understanding of calreticulin's biological significance.
