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Updated: May 17, 2026
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Published on: February 21, 2014
Transcription regulation network analysis of MCF7 breast cancer cells exposed to estradiol
Jun-Zhao Wu1, Peng Lu, Rong Liu
1Breast Cancer Diagnosis and Treatment Center, Henan Cancer Hospital, Henan, China.
Background:
In breast cancer, estrogen receptors have been demonstrated to interact with transcription factors to regulate target gene expression. However, high-throughput identification of the transcription regulation relationship between transcription factors and their target genes in response to estradiol is still in its infancy.
Purpose:
Thus, the objective of our study was to interpret the transcription regulation network of MCF7 breast cancer cells exposed to estradiol.
Methods:
In this work, GSE11352 microarray data were used to identify differentially expressed genes (DEGs).
Results:
Our results showed that the MYB (v-myb myeloblastosis viral oncogene homolog [avian]), PGR (progesterone receptor), and MYC (v-myc myelocytomatosis viral oncogene homolog [avian]) were hub nodes in our transcriptome network, which may interact with ER and, in turn, regulate target gene expression. MYB can up-regulate MCM3 (minichromosome maintenance 3) and MCM7 expression; PGR can suppress BCL2 (B-cell lymphoma 2) expression; MYC can inhibit TGFB2 (transforming growth factor, beta 2) expression. These genes are associated with breast cancer progression via cell cycling and the TGFβ signaling pathway.
Conclusion:
Analysis of transcriptional regulation may provide a better understanding of molecular mechanisms and clues to potential therapeutic targets in the treatment of breast cancer.
Insights
This study deciphers the estradiol-induced transcription regulation network in breast cancer cells. Key transcription factors like MYB, PGR, and MYC were identified as crucial regulators of target genes involved in cancer progression.
Area of Science:
- Molecular Biology
- Genomics
- Cancer Research
Background:
- Estrogen receptors (ER) interact with transcription factors to control gene expression in breast cancer.
- High-throughput methods for identifying estradiol-responsive transcription factor-target gene relationships are underdeveloped.
Purpose of the Study:
- To elucidate the transcription regulation network in MCF7 breast cancer cells upon estradiol exposure.
- To identify key transcription factors and their target genes influenced by estradiol.
Main Methods:
- Utilized GSE11352 microarray data to identify differentially expressed genes (DEGs).
- Analyzed gene expression patterns to construct a transcriptome-wide regulatory network.
Main Results:
- Identified MYB, PGR, and MYC as hub nodes in the estradiol-responsive transcriptome network.
- MYB up-regulates MCM3 and MCM7; PGR suppresses BCL2; MYC inhibits TGFB2.
- These interactions are linked to breast cancer progression through cell cycling and TGFβ signaling.
Conclusions:
- Transcriptional regulation analysis offers insights into breast cancer molecular mechanisms.
- Identified regulatory relationships may suggest potential therapeutic targets for breast cancer treatment.
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