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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: May 17, 2026

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
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Adipose tissue-specific PPARγ gene targeting.

Weimin He1

  • 1Center for Cardiovascular Diseases, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA. hewe@tsu.edu

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2012
PubMed
Summary

Peroxisome proliferator activated receptor gamma (PPARγ) is crucial for fat cell function. Disrupting PPARγ in mature fat cells in mice reveals its in vivo physiological importance.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Peroxisome proliferator activated receptor gamma (PPARγ) is a nuclear receptor highly expressed in adipose tissue.
  • PPARγ is known to regulate adipogenesis and lipogenesis in vitro and in whole organisms.
  • Its specific role in the physiological functions of mature adipocytes in vivo remains incompletely understood.

Purpose of the Study:

  • To investigate the in vivo physiological significance of PPARγ in mature adipocytes.
  • To develop a genetic model for studying PPARγ function specifically in differentiated fat cells.

Main Methods:

  • Utilized Cre-loxP gene targeting strategy for specific gene disruption.
  • Generated "floxed" PPARγ mice with loxP sites flanking critical exons.

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  • Created aP2-Cre transgenic mice expressing Cre recombinase under adipocyte-specific promoter (aP2).
  • Crossed these mouse lines to achieve PPARγ deletion exclusively in mature adipocytes of resulting offspring.

    • Main Results:

    • Successfully generated mice with PPARγ specifically deleted in mature adipocytes.
    • This genetic manipulation allows for the study of PPARγ's role in established fat cells.

    Conclusions:

    • The developed mouse model enables the precise investigation of PPARγ's function in vivo within mature adipocytes.
    • This research will elucidate the receptor's contribution to the physiological regulation of adipose tissue.