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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
The X proteins of bornaviruses interfere with type I interferon signalling
Jonas Johansson Wensman1,2, Muhammad Munir2, Srinivas Thaduri2
1Swedish University of Agricultural Sciences, Department of Clinical Sciences, Division of Ruminant Medicine and Veterinary Epidemiology, PO Box 7054, SE-750 07 Uppsala, Sweden.
Abstract:
Borna disease virus (BDV) is a neurotropic, negative-stranded RNA virus causing persistent infection and progressive neurological disorders in a wide range of warm-blooded animals. The role of the small non-structural X protein in viral pathogenesis is not completely understood. Here we investigated whether the X protein of BDV and avian bornavirus (ABV) interferes with the type I interferon (IFN) system, similar to other non-structural proteins of negative-stranded RNA viruses. In luciferase reporter assays, we found that the X protein of various bornaviruses interfered with the type I IFN system at all checkpoints investigated, in contrast to previously reported findings, resulting in reduced type I IFN secretion.
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