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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
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L-glutaminase production by Trichoderma koningii under solid-state fermentation.

Ashraf S A El-Sayed1

  • 1Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt.

Indian Journal of Microbiology
|October 27, 2012
PubMed
Summary

This study optimized L-glutaminase production using Trichoderma koningii and wheat bran via solid state fermentation. Optimized conditions significantly boosted enzyme yield and productivity compared to submerged methods.

Keywords:
Filamentous fungiL-glutaminaseSolid state fermentationTrichoderma koningii

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Area of Science:

  • Biotechnology
  • Enzyme Production
  • Microbial Fermentation

Background:

  • L-glutaminase is an enzyme with therapeutic applications.
  • Agro-industrial byproducts offer sustainable substrates for microbial fermentation.
  • Solid state fermentation (SSF) can enhance enzyme production.

Purpose of the Study:

  • To optimize solid state fermentation conditions for L-glutaminase production by Trichoderma koningii.
  • To evaluate the efficacy of various agro-industrial byproducts as substrates.
  • To compare SSF productivity with submerged fermentation.

Main Methods:

  • Solid state fermentation using Trichoderma koningii Oud.aggr. on various agro-industrial byproducts.
  • Optimization of fermentation parameters including substrate, moisture content, pH, supplements, inoculum, and incubation time.
  • Assay of L-glutaminase activity, productivity, and yield.

Main Results:

  • Wheat bran was identified as the most effective substrate for L-glutaminase induction.
  • Optimal conditions included 70% moisture, pH 7.0, 1.0% D-glucose, 2.0% L-glutamine, 3 ml inoculum, and 7 days incubation at 30°C.
  • Optimized SSF achieved a productivity of 23.2 U/mg protein and a yield of 45.0 U/gds.
  • Productivity increased 2.2-fold compared to submerged fermentation.

Conclusions:

  • Wheat bran is a suitable and cost-effective substrate for L-glutaminase production by T. koningii using SSF.
  • Optimization of SSF parameters significantly enhances L-glutaminase productivity.
  • Solid state fermentation offers a superior alternative to submerged fermentation for L-glutaminase production by this fungus.