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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
Published on: October 25, 2018
Sequence analysis of a salt tolerant metagenomic clone
Raj Kishor Kapardar1, Ravi Ranjan, Munish Puri
1Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), Mall Road, Delhi, 110 007 India ; Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002 India.
Indian Journal of Microbiology
|October 27, 2012
Summary
Novel genes were discovered in a salt-tolerant metagenomic clone (6B4) from pond water. The ATP-dependent Clp protease adaptor protein (ClpS) encoded by this clone is proposed to confer salt tolerance.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Metagenomes offer a vast source for novel gene discovery.
- Environmental microbes possess unique adaptations, such as salt tolerance.
Purpose of the Study:
- To analyze a salt-tolerant metagenomic clone (6B4) for novel gene identification.
- To investigate the genetic basis of salt tolerance in the identified clone.
Main Methods:
- Sequencing and analysis of a 2254 bp DNA insert from metagenomic clone 6B4.
- Homology searches to identify related organisms and encoded proteins.
- Bioinformatic analysis to predict protein functions.
Main Results:
- Clone 6B4, with 64.06% G+C content, showed homology to proteobacteria.
- Identified encoded proteins include ATP-dependent Clp protease adaptor protein (ClpS), phasin, poly-3-hydroxybutyrate synthase, and permease.
- The Clp complex is known for its role in stress tolerance.
Conclusions:
- The DNA insert in clone 6B4 likely originates from an uncharacterized proteobacterium.
- The expression of ClpS from clone 6B4 is proposed as the mechanism for its observed salt tolerance.
