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Related Experiment Video

Updated: May 17, 2026

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

Elisa based diagnostic test for Aspergillosis.

G L Sharma¹, P U Sarma

¹Centre for Biochemical Technology, University Campus, Mall Road, 110007 Delhi.

Indian Journal of Clinical Biochemistry : IJCB

|October 27, 2012

Summary

This study developed a highly sensitive ELISA test for detecting antibodies in Aspergillosis, improving diagnosis of Aspergillus infections. A convenient diagnostic kit was also formulated and validated for clinical use.

Area of Science:

Medical Microbiology
Immunology
Diagnostic Development

Background:

Accurate diagnosis of Aspergillus-induced infections relies on detecting specific antibodies.
Conventional serological methods like agglutination and electrophoresis lack sensitivity and exhibit high cross-reactivity.

Purpose of the Study:

To characterize and identify diagnostically relevant antigens from Aspergillus fumigatus.
To develop a sensitive and specific diagnostic assay for Aspergillosis using these antigens.
To formulate a user-friendly diagnostic kit for Aspergillus antibody detection.

Main Methods:

Characterization and identification of Aspergillus fumigatus antigens.
Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for detecting specific IgE and IgG antibodies.

Keywords:

Aspergillosis Elisa

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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

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Published on: March 22, 2012

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

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Formulation of a "ready to use" diagnostic kit with dry reagents.

Validation of the ELISA kit using patient sera from various conditions.

Main Results:

Developed an ELISA with 92% sensitivity and 89% specificity for detecting specific IgE and IgG.
Successfully formulated a convenient diagnostic kit with dry reagents.
Validated the kit's satisfactory performance with coded sera from diverse patient groups.

Conclusions:

The developed ELISA offers improved sensitivity and specificity for diagnosing Aspergillosis.
The formulated diagnostic kit provides a practical and effective tool for clinical use.
This advancement aids in the accurate diagnosis of allergic bronchopulmonary aspergillosis, aspergilloma, and invasive aspergillosis.