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Updated: May 17, 2026
Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
Published on: January 12, 2019
Molecularly imprinted electrochemical luminescence sensor based on signal amplification for selective determination
Jianping Li1, Shuhuai Li, Xiaoping Wei
1College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China. likianping@263.net
Abstract:
A new molecularly imprinted electrochemical luminescence (MIP-ECL) sensor was developed for Gibberellin A3 (GA3) determination. This sensor is based on competitive binding between the GA3 and the Rhodamine B (RhB)-labeled GA3 (RhB-GA3) to the MIP film. After the competitive binding, the residual RhB-GA3 on the MIP was electro-oxidized to produce RhB oxide, which could greatly amplify the weak electrochemiluminescence (ECL) signal of luminol. The ECL intensity decreased when the RhB-GA3 was replaced by GA3 molecules in the samples. Accordingly, GA3 was determined in the concentration range from 1 × 10(-11) to 3 × 10(-9) mol/L with a detection limit of 3.45 × 10(-12) mol/L. The sensor shows high sensitivity and selectivity, wide response range, good accuracy, and fast response. Beer samples were assayed by using the sensors, and the recoveries ranging from 96.0% to 103.2% were obtained.
