Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 17, 2026

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Published on: August 9, 2024

AlGaN/GaN high electron mobility transistors for protein-peptide binding affinity study.

Chih-Cheng Huang¹, Geng-Yen Lee, Jen-Inn Chyi

¹Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, 300, Taiwan, ROC.

Biosensors & Bioelectronics

|October 30, 2012

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative Study of DHEA vs. Femarelle® on Menopausal Systemic and Genitourinary Syndrome.

Gynecologic and obstetric investigation·2026

Same author

Prognostic Significance and Immune Landscape of Neuroendocrine Differentiation-Related Genes in Non-Small Cell Lung Cancer.

Biological procedures online·2026

Same author

A Review and Perspective of Techniques for Autonomous Robotic Ultrasound Acquisitions.

Sensors (Basel, Switzerland)·2026

Same author

Towards Feasible Home ECG Monitoring: AI-Driven Detection of Clinically Critical Arrhythmias Using Single-Lead Signals.

Bioengineering (Basel, Switzerland)·2026

Same author

Genetic deletion of miR-200a/200b increases growth and feed conversion efficiency in yellow catfish.

Science China. Life sciences·2026

Same author

A Microfluidic Platform for Viscosity Testing of Non-Newtonian Fluids in Engineering and Biomedical Applications.

Micromachines·2026

This study utilized antibody-functionalized AlGaN/GaN high electron mobility transistors (HEMTs) to detect a short peptide. The research revealed two binding sites on antibodies and accurate dissociation constants, advancing biosensor technology.

Area of Science:

Materials Science
Biotechnology
Electrical Engineering

Background:

High electron mobility transistors (HEMTs) based on Aluminum Gallium Nitride/Gallium Nitride (AlGaN/GaN) heterostructures offer unique electronic properties.
Antibody-immobilized sensors are crucial for detecting biomolecules with high specificity.
Understanding ligand-receptor interactions is fundamental for developing sensitive biosensing platforms.

Purpose of the Study:

To develop and characterize AlGaN/GaN HEMTs functionalized with antibodies for detecting a short peptide.
To analyze the binding kinetics and quantify the interaction parameters between the antibody and the peptide using advanced modeling.
To investigate the limitations of conventional methods for determining dissociation constants in multi-site binding scenarios.

Main Methods:

More Related Videos

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 17, 2026

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Published on: August 9, 2024

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Immobilization of antibodies onto AlGaN/GaN HEMTs to create a biosensor.
Detection of a 20-amino acid short peptide using electrical signal measurements.
Application of one-binding-site and two-binding-site models for signal analysis.
Analysis of surface coverage ratio to correlate with electrical signals.

Main Results:

Successful detection of the short peptide using the antibody-immobilized HEMTs.
Identification of two binding sites on the antibody.
Extraction of two dissociation constants: 4.404×10⁻¹¹ M and 1.596×10⁻⁹ M.
Demonstration that conventional methods may yield inaccurate dissociation constants for multi-site interactions.
Achieved a limit of detection (LOD) of approximately 10 pM, closely matching theoretical predictions.

Conclusions:

AlGaN/GaN HEMTs are effective platforms for highly sensitive biosensing applications.
The study provides valuable insights into antibody-peptide binding affinities and kinetics.
The developed sensor and analysis methods are applicable for biological affinity studies beyond simple detection.