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Updated: May 17, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Detailed structural analysis of the O-polysaccharide expressed by Burkholderia thailandensis E264
Christian Heiss1, Mary N Burtnick, Ian Black
1Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA. cheiss@ccrc.uga.edu
Abstract:
O-polysaccharide (OPS) was isolated from purified Burkholderia thailandensis E264 lipopolysaccharide by mild-acid hydrolysis and gel-permeation chromatography. Glycosyl composition and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy experiments revealed that the OPS antigen was an unbranched heteropolymer with the following structure: [structure: see text] Collectively, these results suggest that B. thailandensis OPS is structurally more complex than B. pseudomallei OPS and provide evidence of the signal used by B. thailandensis to terminate chain elongation.
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