Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Diabetic Nephropathy

Diabetic Nephropathy

Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...

The Extracellular Matrix

The Extracellular Matrix

The Extracellular Matrix

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...

Extracellular Matrix

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

Overview of Cell-Matrix Interactions

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Results of an international, randomized trial comparing glucose metabolism disorders and outcome with cyclosporine versus tacrolimus. Am J Transplant 7 1506-1514, 2007.

Clinical journal of the American Society of Nephrology : CJASN·2023

Same author

Maternal body mass index and placental weight: a role for fetal insulin, maternal insulin and leptin.

Journal of endocrinological investigation·2022

Same author

Short-term perioperative parecoxib is not detrimental to shaft fracture healing in a rat model.

Bone & joint research·2019

Same author

Marine n-3 Polyunsaturated Fatty Acid Supplementation and Quality of Life After Kidney Transplant.

Transplantation proceedings·2019

Same author

Pancreas transplant rejection episodes are not revealed by biopsies of the donor duodenum in a prospective study with paired biopsies.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2018

Same author

Outcomes in Pancreas Transplantation With Exocrine Drainage Through a Duodenoduodenostomy Versus Duodenojejunostomy.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017

Search research articles

Related Experiment Video

Updated: May 17, 2026

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Published on: October 13, 2018

Diabetic nephropathy and extracellular matrix.

S O Kolset¹, F P Reinholt, T Jenssen

¹Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Oslo, Norway. s.o.kolset@medisin.uio.no

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society

|October 30, 2012

Summary

This summary is machine-generated.

Diabetic nephropathy (DN) involves extracellular matrix (ECM) changes, particularly proteoglycans (PGs). Understanding these ECM alterations is key to identifying early diabetic kidney disease markers.

More Related Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Related Experiment Videos

Last Updated: May 17, 2026

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Published on: October 13, 2018

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Area of Science:

Nephrology
Diabetology
Biochemistry

Background:

Diabetic nephropathy (DN) is a severe diabetes complication.
Morphological changes in DN stem from extracellular matrix (ECM) alterations.
ECM expansion in basement membranes, mesangium, and tubulointerstitial space is characteristic of DN.

Purpose of the Study:

To explore the complex changes in proteoglycans (PGs) and their role in DN.
To investigate the involvement of enzymes modulating ECM components in DN.
To assess the potential of ECM components as early biomarkers for diabetic kidney disease.

Main Methods:

Analysis of ECM component alterations in DN.
Examination of proteoglycan (PG) distribution and heparan sulfate chain modifications.
Investigation of enzyme activities (heparanase, sulfatases, MMPs, plasminogen activator) and their inhibitors in DN.

Main Results:

PGs show differential changes: decreased in basement membranes, increased in mesangium and tubulointerstitial space.
Heparan sulfate structure modifications impact growth factor levels and cell interactions.
Enzymes like heparanase, sulfatases, MMPs, and plasminogen activator system components are altered in DN kidneys and plasma.

Conclusions:

ECM remodeling, especially PGs and heparan sulfate, is central to DN pathogenesis.
Enzymatic modulation of ECM is significantly implicated in DN progression.
Further research is needed to establish ECM components as reliable early clinical markers for DN.