Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 17, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Label-acquired magnetorotation for biosensing: An asynchronous rotation assay.

Ariel Hecht¹, Paivo Kinnunen, Brandon McNaughton

¹The University of Michigan, Department of Biomedical Engineering, 2200 Bonisteel, Ann Arbor, MI 48109-2099, United States ; The University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, MI 48109-1055, United States.

Journal of Magnetism and Magnetic Materials

|October 30, 2012

Summary

This study introduces label-acquired magnetorotation (LAM), a new biosensing technique using magnetic particles. LAM measures analyte concentration by detecting the rotation frequency of magnetic bead complexes, demonstrating a two-order-of-magnitude dynamic range.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Insights into the mechanism of nitrate salt-mediated MgCO<sub>3</sub> formation.

Physical chemistry chemical physics : PCCP·2025

Same author

Targeted 8-arm PEG Nanosystems for Localization of Choroidal Neovascularization Macular Degeneration Model.

ACS applied bio materials·2024

Same author

Photoacoustic lifetime oxygen imaging of radiotherapy-induced tumor reoxygenation <i>In Vivo</i>.

Journal of photochemistry and photobiology·2024

Same author

Impact of NH<sub>4</sub>OH treatment on the ion exchange and pore characteristics of a metakaolin-based geopolymer.

RSC advances·2024

Same author

Photoacoustic Chemical Imaging Sodium Nano-Sensor Utilizing a Solvatochromic Dye Transducer for In Vivo Application.

Biosensors·2023

Same author

Photoacoustic Spectral Analysis for Evaluating the Aggressiveness of Prostate Cancer Labeled by Methylene Blue Polyacrylamide Nanoparticles.

Biosensors·2023

Area of Science:

Biotechnology
Biosensing
Nanotechnology

Background:

Biosensing technologies are crucial for detecting biological molecules.
Existing methods often require complex labeling or have limited dynamic ranges.
Novel approaches are needed to improve sensitivity and efficiency in biological detection.

Purpose of the Study:

To introduce and validate a novel biosensing technique called label-acquired magnetorotation (LAM).
To demonstrate the feasibility of LAM using model analytes (streptavidin and biotin).
To establish the dynamic range of the LAM method for potential biological applications.

Main Methods:

Combines the sandwich assay format with asynchronous magnetic bead rotation (AMBR).
Analyte facilitates binding of magnetic label beads to a nonmagnetic solid phase sphere, forming a sandwich complex.

More Related Videos

Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

Published on: May 2, 2014

Related Experiment Videos

Last Updated: May 17, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

Published on: May 2, 2014

The rotational frequency of the complex in a rotating magnetic field is measured as a function of analyte concentration.

Main Results:

Successfully demonstrated the label-acquired magnetorotation (LAM) principle using model analytes.
The developed LAM method exhibits a dynamic range of two orders of magnitude.
The rotational frequency of the sandwich complex is directly related to the amount of analyte.

Conclusions:

Label-acquired magnetorotation (LAM) is a promising novel biosensing approach.
The method shows potential for quantifying biological targets with a significant dynamic range.
Future work will focus on adapting LAM for detecting actual proteins and other biological targets.