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Related Experiment Video

Updated: May 17, 2026

Fast and Accurate Exhaled Breath Ammonia Measurement

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

A rapid method for plasma ammonia estimation using an indigenously purified enzyme.

M A Qadar Pasha¹, R B Ram, M D Gupta

¹Centre for Biochemical Technology, Mall Road, 110007 Delhi.

Indian Journal of Clinical Biochemistry : IJCB

|October 30, 2012

Summary

This study developed a rapid blood ammonia assay for diagnosing liver disease. The new method is stable, sensitive, and suitable for routine clinical use.

Keywords:

Ammonia deaminase inhibitor enzymatic assay glutamate dehydrogenase

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Published on: January 22, 2018

Area of Science:

Clinical Chemistry
Biochemical Diagnostics
Enzymology

Background:

Accurate determination of blood ammonia levels is crucial for diagnosing hepatic disorders.
Existing methods may require optimization for clinical settings.

Purpose of the Study:

To standardize and validate a novel enzymatic assay for blood ammonia determination.
To assess the assay's performance characteristics and suitability for routine clinical use.

Main Methods:

Utilized an indigenously purified enzyme for assay standardization.
Employed a two-reagent system with a 5-minute reaction time at 25-27°C.
Conducted performance evaluations including linearity, imprecision, functional sensitivity, and interference studies.

Main Results:

The assay demonstrated stability for at least one year with lyophilized reagents.
Plasma ammonia levels were significantly higher in patients with hepatic disorders (69.1±32.4 μMol/L) compared to controls (13.7±7.3 μMol/L).
Functional sensitivity ranged from 2-1000 μMol/L with low coefficients of variation (1.1-3.7%).

Conclusions:

The developed enzymatic assay is reliable, sensitive, and stable for quantifying blood ammonia.
The assay's rapid turnaround time and broad measurement range make it ideal for routine clinical diagnostics of hepatic disorders.