Related Experiment Video

Updated: May 17, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Relationship of plasma homocysteine with lipid profile parameters in ischemic heart disease

A S Yadav1, V R Bhagwat, I M Rathod

  • 1Department of Biochemistry, MIMSR Medical College, 413 531 Latur.

Indian Journal of Clinical Biochemistry : IJCB
|October 30, 2012
PubMed

Insights

Elevated plasma homocysteine levels are linked to ischemic heart disease and myocardial infarction. This suggests a potential increased need for B vitamins like folic acid and B12 to regulate homocysteine metabolism.

Area of Science:

  • Biochemistry
  • Cardiology
  • Nutritional Science

Background:

  • Ischemic heart disease (IHD) is a significant global health concern.
  • Elevated plasma homocysteine is a recognized risk factor for cardiovascular diseases.
  • Understanding the biochemical factors associated with IHD is crucial for developing effective prevention and treatment strategies.

Purpose of the Study:

  • To investigate the association between plasma homocysteine levels and other biochemical markers in patients with ischemic heart disease.
  • To compare plasma homocysteine levels in IHD patients with those in healthy controls.

Main Methods:

  • Plasma total homocysteine levels were quantified using High-Performance Liquid Chromatography (HPLC) with fluorescence detection.
  • A cohort of 60 ischemic heart disease patients (including myocardial infarction and chronic stable IHD) was studied.
  • A control group of 30 age-matched healthy individuals was included for comparison.

Main Results:

  • Patients with both myocardial infarction and chronic stable ischemic heart disease exhibited significantly higher plasma homocysteine levels compared to healthy controls.
  • The observed hyperhomocysteinemia in IHD patients suggests a potential metabolic derangement.

Conclusions:

  • Elevated plasma homocysteine is a significant finding in ischemic heart disease patients.
  • Hyperhomocysteinemia may result from an increased bodily requirement for vitamins (folic acid, B12, B6, B2) essential for homocysteine metabolism.
  • Further research into vitamin supplementation could be beneficial for managing IHD.

Keywords:
High density lipoprotein (HDL)High performance liquid chromatography (HPLC)Homocysteine (Hcy)Ischemic heart disease (IHD)Total cholesterol (TC) and Triglycerides (TG)

Related Concept Videos

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
Blood Studies for Cardiovascular System III: Serum Lipid Profile01:25

Blood Studies for Cardiovascular System III: Serum Lipid Profile

Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Ischemic Heart Disease: Overview01:17

Ischemic Heart Disease: Overview

Ischemic heart disease occurs when the heart's blood supply dwindles, causing an ominous lack of oxygen and nutrients. This deficiency, stemming from reduced or obstructed blood flow, spells danger, leading to heart muscle damage and dysfunction.
Atherosclerosis, the primary malefactor, orchestrates this dangerous condition. It manifests as the accumulation of fatty deposits, akin to insidious plaques, within arterial walls. As time elapses, these plaques metamorphose, hardening and narrowing...
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow01:26

Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow

Chronic liver disease significantly impacts drug metabolism due to alterations in hepatic blood flow and enzyme accessibility. This disruption affects the body's pharmacokinetics—the movement and processing of drugs within the system. Key enzymes crucial for metabolizing medications become less accessible, changing how drugs are processed and utilized. Furthermore, liver disease influences the synthesis of plasma proteins, such as albumin and globulins, which play critical roles in drug binding...
Lipid-Lowering Drugs: Statins and Miscellaneous Agents01:20

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...