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Updated: May 17, 2026
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Published on: March 4, 2017
Stability of total and free prostate specific antigen in serum submitted to intermittent cold storage conditions
Asmahan A El Ezzi1, Mohammed A El-Saidi
1Laboratory of Radioimmunoassay, Lebanese Atomic Energy Commission (LAEC), National Council for Scientific Research (NCSR), Airport Avenue, B.P. 11-8281, Beirut, Lebanon ; Rafik Hariri Campus, Lebanese University, College of Science, Biochemistry department, Hadath, Lebanon ; Laboratory of Radioimmunoassay, Lebanese Atomic Energy Commission, NCSR, Airport Avenue, B.P. 11-8281, Beirut, Lebanon.
Abstract:
The goal of this study was to determine the stability of Total Prostrate Specific antigen (PSA-T) and Free Prostrate Specific Antigen (PSA-F) in archival serum stored at 4°C and -20°C and subjected to temperature shift due to interruption in power supply. Our study showed that PSA-T was stable up to 285 days and PSA-F was stable for 158 days under these conditions. Since power supply interruption is an unavoidable problem in developing nations, our study has implication on the validity of measurement of PSA-T and PSA-F in serum that was not properly stored due to emergency situations and for certain types of retrospective studies.
