Protein crystallization and biosensor applications of hydrogel-based molecularly imprinted polymers
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Published on: February 16, 2018
Subrayal M Reddy1, Quan T Phan, Hazim El-Sharif
1Department of Chemistry, Faculty of Engineering and Physical Sciences, University of Surrey, GU2 7XH, United Kingdom. s.reddy@surrey.ac.uk
Abstract:
We have characterized the imprinting capability of a family of acrylamide polymer-based molecularly imprinted polymers (MIPs) for bovine hemoglobin (BHb) and trypsin (Tryp) using spectrophotometric and quartz crystal microbalance (QCM) sensor techniques. Bulk gel characterization on acrylamide (AA), N-hydroxymethylacrylamide (NHMA), and N-isopropylacrylamide (NiPAM) gave varied selectivities when compared with nonimprinted polymers. We have also harnessed the ability of the MIPs to facilitate protein crystallization as a means of evaluating their selectivity for cognate and noncognate proteins. Crystallization trials indicated improved crystal formation in the order NiPAM
