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Updated: May 17, 2026

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation
Published on: September 28, 2022
A correlative model to predict in vivo AUC for nanosystem drug delivery with release rate-limited absorption
Mohammad Barzegar-Jalali1, Kaivan Mohammadi, Ghobad Mohammadi
1Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Purpose:
Drug release from nanosystems at the sites of either absorption or effect biophase is a major determinant of its biological action. Thus, in vitro drug release is of paramount importance in gaining insight for the systems performance in vivo.
Methods:
A novel in vitro in vivo correlation, IVIVC, model denoted as double reciprocal area method was presented and applied to 19 drugs from 55 nano formulations with total 336 data, gathered from literature.
Results:
The proposed model correlated the in vitro with in vivo parameters with overall error of 12.4 ± 3.9%. Also the trained version of the model predicted the test formulations with overall error of 15.8 ± 3.7% indicating the suitability of the approach. A theoretical justification was provided for the model considering the unified classical release laws.
Conclusion:
The model does not necessitate bolus intravenous drug data and seems to be suitable for IVIVC of drugs with release rate-limited absorption.
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