Related Experiment Video
Updated: May 17, 2026
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
Imaging analysis of early DMP1 mediated dentine remineralization
Ana K Bedran-Russo1, Sriram Ravindran, Anne George
1Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina street, Chicago, IL 60612, USA. bedran@uic.edu
Objective:
This study assessed the micro-morphological changes in demineralized dentine scaffold following incubation with recombinant dentine matrix protein 1 (rDMP1).
Design:
Extracted human molar crowns were sectioned into 6 beams (dimensions: 0.50mm×1.70mm×6.00mm), demineralized and incubated overnight in 3 different media (n=4): rDMP1 in bovine serum albumin (BSA), BSA and distilled water. Samples were placed in a chamber with simulated physiological concentrations of calcium and phosphate ions at constant pH 7.4. Samples were immediately processed for transmission electron microscopy (TEM) and field emission-scanning electron microscopy (FE-SEM) after 1 and 2 weeks.
Results:
Analysis of the scaffold showed that decalcification process retained the majority of endogenous proteoglycans and phosphoproteins. rDMP1 treated samples promoted deposition of amorphous calcium phosphate (ACP) precursors and needle shaped hydroxyapatite crystals surrounding collagen fibrils. The BSA group presented ACP bound to collagen with no needle-like apatite crystals. Samples kept in distilled water showed no evidence of ACP and crystal apatite. Results from rDMP1 immobilized on dentine matrix suggests that the acidic protein was able to bind to collagen fibrils and control formation of amorphous calcium phosphate and its subsequent transformation into hydroxyapatite crystals after 2 weeks.
Conclusion:
These findings suggest a possible bio-inspired strategy to promote remineralization of dentine for reparative and regenerative purposes.
