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Eleven microsatellite markers for the mountain clover Trifolium montanum (Fabaceae)
Philippe Matter1, Kirsti Määttänen, Chris J Kettle
1Institute of Terrestrial Ecosystems (ITES)-Ecosystem Management, Building CHN Floor G, ETH Zürich, Universitaetstrasse 16, 8092 Zürich, Switzerland. philippe.matter@env.ethz.ch
Premise Of The Study:
Microsatellite markers were developed in Trifolium montanum to investigate pollen dispersal patterns at landscape scale with a pollen-pool analysis (indirect parentage analysis) as well as in an experimental set-up with a direct paternity analysis.
Methods And Results:
Screening 46 microsatellites developed for T. repens yielded four markers usable in T. montanum. Seven additional ones have been developed specifically for the target species using a 454-sequencing approach. All markers were polymorphic, with an allele number ranging from two to 45 based upon 254 individuals sampled from four populations, and an exclusion probability of 0.999.
Conclusions:
These markers proved a useful and reliable molecular tool for use in population genetics and parentage studies of this common grassland herb.
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