Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

Column Efficiency: Rate Theory

Column Efficiency: Rate Theory

The rate theory of chromatography provides quantitative insight into the shapes and widths of elution bands. These bands are based on the random-walk mechanism governing molecular migration within a column. The Gaussian profile of chromatographic bands arises from the cumulative effect of random molecular motions as they progress through the column.
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Analyte Adsorption and Distribution

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model

Various dissolution theories provide insight into the factors that influence the dissolution rate. Danckwerts' Model suggests that turbulence, rather than a stagnant layer, characterizes the dissolution medium at the solid-liquid interface. In this model, the agitated solvent contains macroscopic packets that move to the interface via eddy currents, facilitating the absorption and delivery of the drug to the bulk solution. The regular replenishment of solvent packets maintains the concentration...

The Integrated Rate Law: The Dependence of Concentration on Time

The Integrated Rate Law: The Dependence of Concentration on Time

While the differential rate law relates the rate and concentrations of reactants, a second form of rate law called the integrated rate law relates concentrations of reactants and time. Integrated rate laws can be used to determine the amount of reactant or product present after a period of time or to estimate the time required for a reaction to proceed to a certain extent. For example, an integrated rate law helps determine the length of time a radioactive material must be stored for its...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Unveiling catalytic potential and the native role of PETase from Streptomyces sp.

Protein science : a publication of the Protein Society·2026

Same author

The SPI-20 and SPI-21 T6SS gene clusters from <i>Salmonella enterica</i> subspecies <i>arizonae</i> encode effector proteins that display antibacterial activity.

Frontiers in microbiology·2026

Same author

Geometric deep learning assists protein engineering. Opportunities and Challenges.

Biotechnology advances·2025

Same author

Metabolic Engineering of Streptomyces leeuwenhoekii C34<sup>T</sup> to Increase Chaxamycin Production Based on the iVR1007 Genome-Scale Model.

Biotechnology and bioengineering·2025

Same author

Production of squalene and fatty acids by Thraustochytrium sp. RT2316-16: effects of dissolved oxygen and the medium composition.

Bioresources and bioprocessing·2025

Same author

Expression and characterization of cold-adapted xylanase Xyl-L in Pichia pastoris for xylooligosaccharide (XOS) preparation.

Microbial cell factories·2025

Search research articles

Related Experiment Video

Updated: May 17, 2026

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Elution relationships to model affinity chromatography using a general rate model.

Gabriela Sandoval¹, Barbara A Andrews, Juan A Asenjo

¹University of Chile, Santiago, Chile.

Journal of Molecular Recognition : JMR

|October 31, 2012

Summary

This summary is machine-generated.

This study models affinity chromatography using a general rate model, comparing power law and exponential elution relationships for protein separation. The exponential model better simulates symmetrical peaks, while the power law excels with asymmetrical ones.

More Related Videos

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

Related Experiment Videos

Last Updated: May 17, 2026

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

Area of Science:

Biochemical Engineering
Separation Science
Mathematical Modeling

Background:

Affinity chromatography is crucial for protein purification.
Existing mathematical models vary in complexity.
Understanding elution kinetics is key for optimizing separation.

Purpose of the Study:

To investigate a general rate model for affinity chromatography.
To evaluate the impact of mobile phase properties and protein affinity on simulations.
To compare power law and exponential elution relationships for protein mixtures.

Main Methods:

Utilized a general rate model incorporating axial dispersion, mass transfer, diffusion, and kinetics.
Simulated two systems: Blue Sepharose with salt gradient and Protein A with pH gradient.
Obtained kinetic parameters for power law and exponential elution models.

Main Results:

Achieved good agreement between experimental and simulated elution curves.
Demonstrated that exponential elution models best represent symmetrical protein peaks.
Showed that power law elution models are more suitable for asymmetrical protein peaks.

Conclusions:

The choice of elution relationship significantly impacts affinity chromatography simulations.
The general rate model provides accurate predictions for protein elution.
Selecting the appropriate kinetic model enhances the predictability of protein separation processes.