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Updated: May 17, 2026

Construction of Out-of-Equilibrium Metabolic Networks in Nano- and Micrometer-Sized Vesicles
Published on: April 12, 2024
Giant vesicles as encapsulating matrix for stabilizing alcohol oxidase and as container for coupled enzymatic
Sabitoj S Virk1, Vishwa J Baruah, Pranab Goswami
1Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. sabitoj@alumni.iitg.ernet.in
Abstract:
Encapsulation of a multimeric alcohol oxidase (AOx) in giant vesicles (GVs) was prepared by rapid evaporation method. The specific activity (477 U/mg-protein) of the GV encapsulated AOx was 3.5 fold higher than the free AOx. The half-life (˜59.4 hours) of the encapsulated AOx was 20-fold higher than the free enzyme at 30°C. Further, the GV was used to encapsulate AOx and HRP together as coupled reaction catalyst for chromogen-based optical detection of alcohols. The results confirmed potential application of the GV as micro-dimensional container for enhancing stability, activity and biosensing applications of AOx.
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