Linking predator-prey interactions with exposure to a trophically transmitted parasite using PCR-based analyses

Area of Science:

• Ecology
• Parasitology
• Molecular Biology

Background:

• Parasite transmission depends on host contact rates and susceptibility.
• Trophically transmitted parasites rely on host feeding behavior for transmission.
• Measuring exposure variation in natural populations is challenging.

Purpose of the Study:

• Investigate temporal and individual variations in parasite exposure.
• Determine the role of host diet in parasite transmission.
• Identify factors influencing infection risk in white-footed mice (Peromyscus leucopus).

Main Methods:

• Combined epidemiological data with molecular analyses (polymerase chain reaction - PCR).
• Analyzed the diet of white-footed mice to detect intermediate host DNA.
• Correlated host diet with infection status.

Main Results:

• Cricket consumption was a major driver of infection risk, increasing likelihood by fourfold.
• Pregnant female mice consumed more crickets and had threefold higher infection rates.
• Male infection rates were higher in breeding condition, suggesting differential susceptibility beyond diet.

Conclusions:

• Host diet heterogeneity is a strong predictor of parasite exposure.
• Individual susceptibility, especially in males, may also influence infection dynamics.
• Combining molecular and epidemiological data reveals complex patterns in parasite transmission.