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Updated: May 17, 2026
Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
Published on: May 29, 2017
Detection of trace processes in the networks of neurons cultured on microelectrode arrays
A L Sukhanova1, O A Mineyeva, I I Kiselev
1Department of Neuroscience, NBICS Center, National Research Center Kurchatov Institute, Laboratory of Neurobiology of Memory, PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia. alsukhanova@gmail.com
Abstract:
We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode arrays were exposed to two stimulation protocols: high-frequency and low-frequency stimulations. Expression of c-fos was evaluated using immunofluorescence. Both high-frequency and low-frequency stimulations significantly increased c-fos expression in comparison with non-stimulated control. These findings indicate that c-fos expression can be induced in neuronal cell culture by different types of electrical stimulations and can be used for studying plasticity processes in microphysiological in vitro systems.
