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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Published on: January 16, 2018

Single-molecule localization super-resolution microscopy: deeper and faster.

Sébastien Herbert1, Helena Soares, Christophe Zimmer

  • 1Institut Pasteur, Groupe Imagerie et Modélisation, CNRS URA 2582, 25 rue du Docteur Roux, 75015 Paris, France.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|November 2, 2012
PubMed
Summary
This summary is machine-generated.

Single-molecule localization microscopy offers super-resolution imaging with nanometer accuracy, surpassing conventional limits. Recent advancements in labeling, hardware, and analysis are driving its accessibility and application in biology.

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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Molecular Imaging

Background:

  • Conventional microscopy is limited by diffraction (≈200 nm lateral, ≈500 nm axial).
  • Single-molecule localization microscopy (SMLM) has achieved nanometer-level precision for over a decade.
  • Emergence of SMLM techniques is driven by advances in fluorescence labeling, imaging hardware, and image analysis.

Purpose of the Study:

  • To review the historical development of SMLM.
  • To summarize recent breakthroughs in SMLM.
  • To discuss potential future applications of SMLM.

Main Methods:

  • Photoactivated localization microscopy (PALM)
  • Stochastic optical reconstruction microscopy (STORM)
  • Minimal modifications to conventional fluorescence microscopes (wide-field, TIRF)

Main Results:

  • PALM and STORM offer a tenfold resolution increase over standard microscopy.
  • These methods are increasingly accessible due to commercialization.
  • Ongoing evolution aims for high temporal resolution and depth imaging, including live samples.

Conclusions:

  • SMLM represents a significant leap in biological imaging resolution.
  • Commercial availability is accelerating research adoption.
  • Future developments promise enhanced capabilities for dynamic and deep-tissue imaging.