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Counting Proteins in Single Cells with Addressable Droplet Microarrays
Published on: July 6, 2018
DropletMicroarray: facile formation of arrays of microdroplets and hydrogel micropads for cell screening applications
Erica Ueda1, Florian L Geyer, Victoria Nedashkivska
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Postfach 3640, 76021 Karlsruhe, Germany.
Lab on a Chip
|November 2, 2012
Abstract:
We describe a one-step method for creating thousands of isolated pico- to microliter-sized droplets with defined geometry and volume. Arrays of droplets are instantly formed as liquid moves along a superhydrophilic-superhydrophobic patterned surface. Bioactive molecules, nonadherent cells, or microorganisms can be trapped in the fully isolated microdroplets for high-throughput screening, or in hydrogel micropads for screening in 3D microenvironments.
