Related Experiment Video

Updated: May 17, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne

Michele T Jay-Russell1, Robert E Mandrell, Jean Yuan

  • 1University of California, Davis, Davis, California, USA. mjay@ucdavis.edu

Journal of Clinical Microbiology
|November 2, 2012
PubMed

Insights

Major outer membrane protein (MOMP) typing effectively screened Campylobacter jejuni strains from dairy farms linked to milk-borne outbreaks. This method identified environmental sources of campylobacteriosis, aiding in public health investigations.

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Campylobacter jejuni is a leading cause of bacterial gastroenteritis globally.
  • Milk-borne transmission of C. jejuni poses a significant public health risk.
  • Accurate source tracking of C. jejuni outbreaks is crucial for effective control.

Purpose of the Study:

  • To evaluate major outer membrane protein (MOMP) typing as a screening tool for Campylobacter jejuni.
  • To compare the porA gene sequences of clinical outbreak strains with dairy farm environmental strains.
  • To confirm the genetic relatedness of clinical and environmental C. jejuni isolates.

Main Methods:

  • Comparison of Campylobacter jejuni porA gene sequences using major outer membrane protein (MOMP) typing.
  • Confirmation of genetic relatedness through multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).
  • Isolation and characterization of C. jejuni from bovine fecal, water, and lagoon samples at two dairies.

Main Results:

  • MOMP typing successfully identified C. jejuni strains with identical or closely related porA sequences between clinical and environmental samples.
  • Environmental C. jejuni strains indistinguishable from clinical outbreak strains were isolated from flush alley water and bovine fecal samples at both dairies.
  • The study linked two distinct milk-borne campylobacteriosis outbreaks in California to specific dairy farm environments.

Conclusions:

  • Major outer membrane protein (MOMP) typing is a valuable and efficient screening method for environmental C. jejuni isolates.
  • This approach facilitates the identification of potential sources of milk-borne campylobacteriosis outbreaks.
  • The findings support the use of MOMP typing to prioritize isolates for more intensive molecular subtyping techniques.

More Related Videos

Detection and Isolation of Campylobacter spp. from Raw Meat05:34

Detection and Isolation of Campylobacter spp. from Raw Meat

Published on: February 23, 2024

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach09:31

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach

Published on: October 7, 2025

Related Concept Videos

Investigation of Disease Outbreaks01:23

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...