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Updated: May 17, 2026

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
Published on: May 24, 2020
Fixation of emerging interviral recombinants in cucumber mosaic virus populations
Justin S Pita1, Marilyn J Roossinck
1Department of Plant Pathology and Environmental Biology, and The Huck Institutes of The Life Sciences, Center for Infectious Disease Dynamics, Pennsylvania State University, University Park, PA, USA.
Abstract:
Interstrain recombinants were observed in the progenies of the Cucumber mosaic virus (CMV) reassortant L(1)L(2)F(3) containing RNAs 1 and 2 from LS-CMV and RNA 3 from Fny-CMV. We characterized these recombinants, and we found that their fixation was controlled by the nature of the replicating RNAs 1 and 2. We demonstrate that the 2b gene partially affects this fixation process, but only in the context of homologous RNAs 1 and 2.

