Related Experiment Video
Updated: Aug 18, 2026

Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
Development and properties of a cell culture produced vaccine for hog cholera based on the Chinese strain
C Terpstra1, R Woortmeyer, S J Barteling
1Central Veterinary Institute, Virology Department, Lelystad, The Netherlands.
Abstract:
The Chinese strain of hog cholera virus (HCV) was adapted to suspension cultures of the established swine kidney cell line SK6. The strain designated "Cedipest", is produced on the basis of a seedlot system. The masterseed virus was identified in vitro and in vivo, and was found free from extraneous pig pathogenic viruses by repeated animal inoculation followed by appropriate serological tests. A distinct and reproducible relationship was ascertained between infectivity in vitro and protection. Pigs inoculated with 400-600 TCID50 of the Cedipest strain proved fully protected against challenge with greater than 100 pig LD50 of a virulent strain of HCV at 7 days and at 6 month post vaccination.
More Related Videos
07:58Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
12:53Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
Published on: March 16, 2022
Related Concept Videos
Vaccine Production
Cholera