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Updated: May 17, 2026

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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Synthesis of a targeted biarsenical Cy3-Cy5 affinity probe for super-resolution fluorescence imaging
Na Fu1, Yijia Xiong, Thomas C Squier
1Biological Sciences Division, Fundamental Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.
Journal of the American Chemical Society
|November 3, 2012
Abstract:
Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are of significant interest due to their ability to enable in situ imaging of protein complexes within native biomolecular assemblies. Here we describe the synthesis of a fluorescent probe (AsCy3Cy5) and demonstrate the targeted labeling and super-resolution imaging of a tagged protein within a supramolecular protein complex.

