Related Experiment Video
Updated: May 17, 2026
10:17
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Simultaneous analysis of 17 diuretics in dietary supplements by HPLC and LC-MS/MS
1Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Korea Food & Drug Administration, Osong-eup, Cheongwon-gun, Chungcheongbuk-do, Korea.
Summary
New analytical methods using HPLC and LC-MS/MS can detect 17 diuretics in health foods. These validated techniques confirmed no diuretics were found in tested dietary supplements, ensuring product safety.
Area of Science:
- Analytical Chemistry
- Food Safety
- Pharmacology
Background:
- Dietary supplements are widely used for weight loss.
- Concerns exist regarding the illegal addition of diuretics to these products.
- Accurate analytical methods are crucial for detecting adulterants.
Purpose of the Study:
- To develop and validate simple, rapid, selective, and sensitive methods for the simultaneous analysis of 17 diuretics.
- To test dietary supplements for the presence of illegally added diuretics.
- To ensure the safety and authenticity of health foods.
Main Methods:
- High-Performance Liquid Chromatography (HPLC) with UV detection.
- Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM).
- Method validation including linearity, precision, recovery, and reproducibility.
Main Results:
- Both HPLC and LC-MS/MS methods were successfully validated for analyzing 17 diuretics.
- Sixteen dietary supplements were tested, and diuretics were not detected in any sample.
- Extraction recoveries ranged from 81% to 116%, demonstrating method efficiency across different formulations.
Conclusions:
- The developed HPLC and LC-MS/MS methods are simple, fast, reliable, and suitable for routine screening.
- These methods can effectively monitor for illegal diuretic adulteration in dietary supplements.
- The findings support the safety of the tested dietary supplements regarding diuretic content.
