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Related Experiment Videos

Anisakis simplex: histopathological changes in experimentally infected CBA/J mice.

R E Jones1, T L Deardorff, S G Kayes

  • 1Department of Structural and Cellular Biology, University of South Alabama College of Medicine, Mobile 36688.

Experimental Parasitology
|April 1, 1990
PubMed
Summary

This study shows that Anisakis simplex infection in mice causes significant immune responses, including granuloma formation and changes in white blood cell counts, mimicking human anisakiasis.

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Area of Science:

  • Parasitology
  • Immunology
  • Veterinary Pathology

Background:

  • Anisakis simplex is a nematode parasite causing anisakiasis in humans.
  • Understanding the host-parasite interaction is crucial for diagnosing and treating anisakiasis.

Purpose of the Study:

  • To investigate the host immune response to Anisakis simplex third-stage larvae (L3) in a murine model.
  • To correlate histological and hematological changes with parasite viability over time.

Main Methods:

  • Surgical implantation of Anisakis simplex L3 into CBA/J mice.
  • Necropsy at 7, 14, and 21 days postinfection (PI).
  • Histopathological examination of lesions and hematological analysis of peripheral blood.

Main Results:

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  • Parasites were found in the gut mesentery, with granuloma formation evolving from neutrophil to eosinophil and fibroblast dominance.
  • Host-parasite interface showed granulocytes and multinucleate giant cells.
  • L3 remained viable at 14 days PI but were dead and infiltrated by inflammatory cells by 21 days PI.
  • Infected mice exhibited neutrophilia and decreased peripheral blood eosinophils, returning to normal by 21 days PI.

Conclusions:

  • Surgical implantation of Anisakis simplex L3 in mice effectively models the host immune response observed in human anisakiasis.
  • The study provides a histological and hematological basis for understanding Anisakis simplex infections.