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Related Experiment Video

Updated: May 17, 2026

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

Two-photon sodium imaging in dendritic spines.

Christine R Rose

    Cold Spring Harbor Protocols
    |November 3, 2012
    PubMed
    Summary

    High-resolution sodium imaging using sodium-binding benzofuran isophthalate (SBFI) allows measurement of intracellular sodium (Na+) transients in neurons and glial cells. In situ calibration is crucial for accurate dynamic sodium concentration measurements.

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    Area of Science:

    • Neuroscience
    • Cellular Physiology
    • Biophysics

    Background:

    • Neuronal activity generates significant postsynaptic sodium (Na+) transients.
    • The physiological impact of these Na+ transients is not well understood.
    • Accurate measurement of intracellular sodium ([Na+]i) is essential for studying neuronal function.

    Purpose of the Study:

    • To provide a protocol for high-resolution Na+ imaging in neurons using SBFI.
    • To detail in situ calibration procedures for SBFI measurements.
    • To enable the study of physiological consequences of Na+ transients.

    Main Methods:

    • Two-photon imaging with the fluorescent Na+ indicator SBFI.
    • Introduction of SBFI into individual neurons in acute tissue slices.
    • In situ calibration of SBFI for accurate [Na+]i measurements.

    Main Results:

    • SBFI enables measurement of [Na+]i transients in neuronal dendrites and spines.
    • The technique allows for high spatial resolution determination of [Na+]i transients in glial cells.
    • SBFI is potentially applicable for in vivo Na+ signal measurements.

    Conclusions:

    • High-resolution Na+ imaging with SBFI is a valuable tool for neuroscience research.
    • In situ calibration is critical for obtaining reliable [Na+]i measurements due to differing intracellular properties.
    • This protocol facilitates the investigation of dynamic Na+ changes in various cell types and potentially in vivo.

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    Last Updated: May 17, 2026

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