Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Spare Receptors01:30

Spare Receptors

Some receptors remain unoccupied even when an agonist produces a maximal response. Such empty ones are called spare receptors. In presence of spare receptors the maximum effect of an agonist drug is achieved with fewer than 100% of the receptors being occupied. To determine the presence of spare receptors, scientists often compare the concentration of the drug needed to produce 50% of the maximum effect (EC50) with the concentration of the drug needed to occupy 50% of the receptors (Kd). If the...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kindlin-2 Deletion in Mural Cells Leads to Vascular Instability.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Kindlin-3 phosphorylation is crucial for thrombosis and hemostasis <i>in vivo</i>.

Research and practice in thrombosis and haemostasis·2025
Same author

Role of Kindlin 2 in prostate cancer.

Scientific reports·2024
Same author

Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer.

Matrix biology : journal of the International Society for Matrix Biology·2023
Same author

Identification and interpretation of TET2 noncanonical splicing site intronic variants in myeloid neoplasm patients.

EJHaem·2023
Same author

Parkin ubiquitination of Kindlin-2 enables mitochondria-associated metastasis suppression.

The Journal of biological chemistry·2023

Related Experiment Video

Updated: May 17, 2026

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
07:13

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader

Published on: May 24, 2024

So many plasminogen receptors: why?

Edward F Plow1, Loic Doeuvre, Riku Das

  • 1Department of Molecular Cardiology, Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Cleveland Clinic, Cleveland, OH 44195, USA. plowe@ccf.org

Journal of Biomedicine & Biotechnology
|November 3, 2012
PubMed
Summary

Cells use many plasminogen receptors (Plg-Rs) to activate plasminogen to plasmin, aiding cell migration. This review explores the biological reasons behind the diverse array of Plg-Rs and their roles.

More Related Videos

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

Related Experiment Videos

Last Updated: May 17, 2026

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
07:13

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader

Published on: May 24, 2024

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
06:45

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

Area of Science:

  • Cell biology
  • Biochemistry
  • Molecular biology

Background:

  • Plasminogen and plasmin bind to cell surfaces via plasminogen receptors (Plg-Rs).
  • Over a dozen Plg-Rs have been identified, many facilitating plasminogen activation and protecting plasmin from inhibitors.
  • Localized plasmin activity supports cellular responses, including migration.

Purpose of the Study:

  • To address the question of why numerous different plasminogen receptors exist.
  • To explore the functional redundancy and specificity of Plg-Rs in cellular processes.
  • To understand the biological significance of multiple Plg-Rs on various cell types.

Main Methods:

  • Literature review and synthesis of existing research on plasminogen receptors.
  • Analysis of the structural diversity and functional roles of identified Plg-Rs.
  • Discussion of cellular responses mediated by Plg-Rs, particularly cell migration.

Main Results:

  • Cells express multiple Plg-Rs, and individual Plg-Rs can be found on diverse cell types.
  • Several different Plg-Rs can support the same cellular function, such as inflammatory cell migration.
  • The existence of numerous Plg-Rs suggests complex regulatory mechanisms and diverse functional contributions.

Conclusions:

  • The multiplicity of plasminogen receptors likely reflects specialized roles in regulating localized protease activity for various cellular functions.
  • Understanding the diverse Plg-R repertoire is crucial for comprehending cell signaling and migration.
  • Further research is needed to fully elucidate the specific contributions of each Plg-R to cellular physiology.