Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Clot Retraction and Fibrinolysis

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Introduction to Hemostasis

Introduction to Hemostasis

Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized, and...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Drug combination choices of 5α-reductase inhibitors and α-blockers in patients with benign prostatic hyperplasia: a study based on the FAERS database.

Frontiers in medicine·2026

Same author

Bidirectional Mendelian Randomization and Single‑Cell RNA Sequencing Reveal an NK Cell-Mediated Causal Link Between Endometriosis and Endometrial Cancer.

International journal of women's health·2026

Same author

NQO1-Mediated Anoikis Resistance and Immune Evasion Define a High-Risk Multi-Omic Subtype for Precision Management of T1 High-Grade Bladder Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Efficacy and safety of RC48 alone or combined with PD-1 inhibitors in high-risk non-muscle invasive bladder cancer.

Scientific reports·2026

Same author

RBM15 drives bladder cancer progression through YTHDF2-dependent m6A-mediated regulation of ZO2.

Journal of experimental & clinical cancer research : CR·2026

Same author

Non-Invasive Urine-Based Diagnostic Technologies for Early Bladder Cancer.

Biosensors·2026

Search research articles

Related Experiment Video

Updated: May 17, 2026

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Published on: June 24, 2008

The plasminogen system in regulating stem cell mobilization.

Yanqing Gong¹, Jane Hoover-Plow

¹Joseph J. Jacobs Center for Thrombosis and Vascular Biology and Department of Cardiovascular Medicine, Cleveland Clinic Lerner Research Institute, Cleveland, OH 44195, USA. gongy@ccf.org

Journal of Biomedicine & Biotechnology

|November 3, 2012

Summary

The plasminogen (Plg) system is crucial for mobilizing hematopoietic stem cells (HPSCs) in response to G-CSF. Understanding Plg

More Related Videos

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

Related Experiment Videos

Last Updated: May 17, 2026

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Published on: June 24, 2008

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

Area of Science:

Hematology
Cardiovascular Biology
Cell Biology

Background:

Hematopoietic progenitor and stem cells (HPSCs) are vital for reconstituting hematopoiesis and repairing tissue damage.
Granulocyte-colony stimulating factor (G-CSF) is clinically used for stem cell mobilization, but responses vary.
The plasminogen (Plg) system, a key fibrinolytic pathway, is increasingly recognized for its role in stem cell mobilization.

Purpose of the Study:

To explore the mechanisms by which the plasminogen (Plg) system regulates hematopoietic stem cell (HPSC) mobilization.
To investigate the role of Plg in G-CSF-induced HPSC mobilization from the bone marrow niche.
To identify potential therapeutic targets for improving stem cell mobilization strategies.

Main Methods:

Review of recent evidence on the plasminogen (Plg) system's role in stem cell mobilization.
Focus on stepwise proteolysis and signal transduction pathways involved in HPSC egress.
Discussion of the Plg system's involvement in G-CSF-mediated mobilization.

Main Results:

The plasminogen (Plg) system plays a pivotal role in hematopoietic stem cell (HPSC) mobilization.
Plg influences HPSC egress from the bone marrow niche through proteolysis and signal transduction.
G-CSF-induced HPSC mobilization is significantly modulated by the Plg system.

Conclusions:

The plasminogen (Plg) system is a critical regulator of hematopoietic stem cell (HPSC) mobilization.
Elucidating these mechanisms can lead to novel therapeutic strategies for hematological and cardiovascular diseases.
Targeting the Plg system may enhance stem cell mobilization efficacy in clinical settings.