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Quercetin inhibits degranulation and superoxide generation in PMA stimulated neutrophils

Jana Pečivová1, Tatiana Mačičková, Klára Sviteková

  • 1Institute of Experimental Pharmacology & Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovakia.

Interdisciplinary Toxicology
|November 3, 2012
PubMed

Insights

Quercetin effectively inhibits neutrophil activity, reducing harmful reactive oxygen species and myeloperoxidase release. This suggests quercetin may help resolve inflammation by decreasing neutrophil respiratory burst and degranulation.

Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Activated neutrophils are key sources of myeloperoxidase (MPO) and superoxide (SO), crucial for microbicidal activity but also implicated in tissue damage during inflammation.
  • Excessive reactive oxygen species and MPO release from neutrophils can disrupt apoptosis, exacerbate inflammation, and contribute to various human diseases.

Purpose of the Study:

  • To investigate the inhibitory effects of quercetin on neutrophil functions, specifically superoxide generation and MPO release.

Main Methods:

  • Isolated human neutrophils were stimulated with phorbol 12-myristate 13-acetate (PMA).
  • The dose-dependent effects of quercetin on superoxide (SO) generation and myeloperoxidase (MPO) release were measured.
  • Quercetin's impact on the activity of intact neutrophils was also assessed.

Main Results:

  • Quercetin demonstrated a dose-dependent inhibition of PMA-stimulated superoxide generation in isolated human neutrophils.
  • Quercetin was more potent in inhibiting MPO release compared to SO generation.
  • The activity of intact isolated neutrophils was not affected by quercetin.

Conclusions:

  • Quercetin effectively reduces neutrophil-mediated oxidative stress and degranulation.
  • Results indicate quercetin's potential role in supporting inflammation resolution by modulating neutrophil activity, specifically respiratory burst and degranulation.
Keywords:
myeloperoxidaseneutrophilsquercetinsuperoxide generation

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