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Related Experiment Videos

DNA fingerprints applied to gene introgression in breeding programs.

J Hillel1, T Schaap, A Haberfeld

  • 1Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.

Genetics
|March 1, 1990
PubMed
Summary

DNA fingerprints (DFP) offer a novel method for gene introgression in breeding programs. This approach uses DFP patterns to select for genomic similarity, significantly reducing backcross generations in farm animals and plants.

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Area of Science:

  • Agricultural Science
  • Genetics
  • Animal Breeding
  • Plant Breeding

Background:

  • Gene introgression is crucial for improving livestock and crops.
  • Traditional backcrossing methods are time-consuming and require numerous generations.
  • Efficient selection strategies are needed to accelerate breeding programs.

Purpose of the Study:

  • To propose and evaluate DNA fingerprints (DFP) as a tool for genomic selection (GS) in gene introgression.
  • To demonstrate the association between DFP similarity and genomic similarity.
  • To reduce the number of backcross generations required for successful gene introgression.

Main Methods:

  • Utilizing highly polymorphic DFP loci detected by minisatellite probes.

Related Experiment Videos

  • Employing DFP patterns to select individuals with maximal genomic similarity to the recipient line and minimal similarity to the donor line.
  • Performing genomic selection at BC1, BC2, or both generations.

    • Main Results:

    • DFP patterns can accurately reflect genomic similarity between individuals and breeding lines.
    • The proposed GS approach using DFP significantly reduces the number of backcross generations.
    • Theoretical distributions and variances for donor and recipient genome percentages were presented.

    Conclusions:

    • DFP provides a powerful and efficient method for accelerating gene introgression in breeding programs.
    • This technique enhances the precision of selecting desired genomic contributions from donor and recipient lines.
    • The DFP-based GS strategy offers a valuable advancement for both animal and plant breeding.