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Updated: May 17, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Integration of multiple components in polystyrene-based microfluidic devices part II: cellular analysis.

Kari B Anderson1, Stephen T Halpin, Alicia S Johnson

  • 1Department of Chemistry, Michigan State University, 578 S. Shaw Blvd, East Lansing, Michigan 48824, USA.

The Analyst
|November 3, 2012
PubMed
Summary

Polystyrene microfluidic devices effectively measure cellular nitric oxide, calcium uptake, and catecholamine release. These PS devices offer enhanced cell adhesion and are suitable for diverse cellular assays and automation.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Materials Science

Background:

  • Microfluidic devices are crucial for cellular assays.
  • Polystyrene (PS) is explored as a material for microfluidic device fabrication.
  • Standard detection methods like electrochemical sensing and optical measurements are utilized.

Purpose of the Study:

  • To evaluate polystyrene (PS) devices for microfluidic-based cellular assays.
  • To assess the measurement of cellular uptake, production, and release of analytes.
  • To compare PS device performance with other materials like PDMS.

Main Methods:

  • Utilized fluorescence measurements for nitric oxide (NO) production in endothelial cells.
  • Employed optical determination for calcium (Ca2+) uptake in endothelial cells.

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Last Updated: May 17, 2026

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  • Integrated electrochemical sensing to monitor catecholamine release from PC 12 cells.

    • Main Results:

    • Demonstrated a four-fold increase in NO production upon ATP stimulation.
    • Showed a significant 42% increase in Ca2+ uptake with ionophore stimulation.
    • Achieved successful electrochemical monitoring of catecholamine release (114 ± 11 μM) from PC 12 cells, with better cell adherence on PS compared to PDMS.

    Conclusions:

    • PS microfluidic devices are effective for measuring diverse cellular analytes (NO, Ca2+, catecholamines).
    • PS offers enhanced cell adhesion, rigidity, and amenability to automation for microfluidic applications.
    • PS material enables investigation of a wider range of analytes, including hydrophobic compounds.