Rapid immunostaining in Mohs: current applications and attitudes

James S Trimble1, Basil S Cherpelis

  • 1Department of Dermatology and Cutaneous Surgery, The University of South Florida, Tampa, Florida, USA. jtrimble@health.usf.edu

Abstract

Insights

Most Mohs surgeons find immunohistochemical (IHC) stains reliable for Mohs micrographic surgery (MMS). However, barriers like time, cost, and education limit their widespread adoption, suggesting potential underutilization in training programs.

Area of Science:

  • Dermatology
  • Surgical Pathology
  • Mohs Micrographic Surgery

Background:

  • Recent literature extensively reviews rapid immunostaining in Mohs micrographic surgery (MMS).
  • Limited data exists on current utilization and attitudes towards immunohistochemical (IHC) stains in Mohs surgery.
  • Understanding the practical application and surgeon perception of IHC stains is crucial.

Purpose of the Study:

  • To assess the utilization rates of rapid immunostaining in Mohs surgery.
  • To gauge the attitudes of fellowship-trained Mohs surgeons towards immunohistochemical (IHC) stains.
  • To identify barriers hindering the adoption of IHC stains in MMS.

Main Methods:

  • A 25-question survey was distributed to members of the American College of Mohs Surgery (ACMS).
  • Surveys were administered via multiple channels including SurveyMonkey, email, fax, and Metrofax.
  • Data was collected from a diverse group of fellowship-trained Mohs surgeons.

Main Results:

  • A total of 378 surveys were completed, representing a broad cross-section of ACMS members.
  • The majority of respondents believe IHC stains are reliable for use in Mohs surgery.
  • Common barriers to IHC stain use include time constraints, lack of education, and costs.

Conclusions:

  • Immunohistochemical (IHC) stains are perceived as reliable by most Mohs surgeons.
  • Increased literature activity correlates with a rise in immunostain usage over the past decade.
  • Potential underutilization of IHC staining in fellowship programs warrants further investigation.