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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...

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Related Experiment Video

Updated: May 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Deciphering the epigenetic code: an overview of DNA methylation analysis methods.

Muhammad Umer1, Zdenko Herceg

  • 1Epigenetics Group, International Agency for Research on Cancer IARC, Lyon 69008, France.

Antioxidants & Redox Signaling
|November 6, 2012
PubMed
Summary

DNA methylation analysis has advanced significantly, enabling gene regulation insights. While challenges remain in quantifying degraded DNA and single-cell mapping, new sequencing technologies promise deeper understanding of epigenomics.

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

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Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Related Experiment Videos

Last Updated: May 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Area of Science:

  • Epigenetics and Genomics
  • Molecular Biology
  • Biochemistry

Background:

  • DNA methylation is crucial for gene regulation in eukaryotes, impacting development and disease.
  • Early methods for DNA methylation analysis were limited to quantifying total methylated cytosines.
  • Recent advances include microarray platforms and massively parallel sequencing for methylome analysis.

Purpose of the Study:

  • To provide an overview of DNA methylation analysis methodologies.
  • To highlight recent developments in genome-wide and high-throughput methods.
  • To discuss the relevance of these methods in cancer research, epigenetics, and redox science.

Main Methods:

  • Overview of various DNA methylation analysis techniques.
  • Focus on recent advancements in genome-wide and high-throughput methods.
  • Discussion of methods for identification, quantification, and mapping of DNA methylation.

Main Results:

  • Established methods offer global, locus-specific, or genome-wide methylation data.
  • Advanced approaches like sequencing are improving methylome analysis.
  • Sensitive quantification from degraded DNA and single-cell mapping remain challenging.

Conclusions:

  • Developments in DNA sequencing and 5-hydroxymethylcytosine mapping will enhance epigenomic understanding.
  • The presented methodologies are applicable to cancer research and broader epigenetics.
  • Continued innovation is expected to further unravel the complexities of DNA methylation.