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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Published on: December 12, 2019
Essential role for Notch signaling in restricting developmental plasticity
Nareg J-V Djabrayan1, Nathaniel R Dudley, Erica M Sommermann
1Department of Molecular, Cellular, and Developmental Biology, Neuroscience Research Institute, University of California at Santa Barbara, Santa Barbara, CA 93106, USA.
Genes & Development
|November 6, 2012
Summary
Notch signaling is crucial for cell commitment in C. elegans embryogenesis. It establishes a memory state, preventing cell reprogramming and promoting developmental commitment through a novel mechanism.
Area of Science:
- Developmental Biology
- Cell Signaling
- Genetics
Background:
- Cell plasticity and commitment are fundamental processes in embryonic development.
- Notch signaling pathways regulate diverse cell fate decisions in many organisms.
- Understanding the precise roles of Notch signaling in developmental transitions is crucial.
Purpose of the Study:
- To investigate the role of Notch signaling in the transition from developmental plasticity to cell commitment during Caenorhabditis elegans embryogenesis.
- To elucidate the molecular mechanisms by which Notch signaling influences cell lineage commitment.
- To differentiate the role of Notch in cell fate commitment from its role in cell fate induction.
Main Methods:
- Utilized genetic analysis in Caenorhabditis elegans.
- Investigated the function of GLP-1 and LIN-12 Notch receptors.
- Examined the requirement for the Delta Serrate Lag-3 (DSL-3) ligand.
Main Results:
- Notch signaling, via GLP-1 and LIN-12 receptors, establishes a memory state in the AB cell lineage.
- This memory state prevents forced reprogramming by endoderm-determining factors.
- A novel ligand, Delta Serrate Lag-3 (DSL-3), is required for this commitment-promoting activity, distinct from known ligands.
Conclusions:
- Notch signaling is essential for promoting developmental commitment in C. elegans.
- This commitment mechanism is autonomous to the AB lineage and distinct from canonical Notch-mediated cell fate induction.
- Notch signaling employs a unique pathway to ensure developmental stability and prevent lineage plasticity.
Related Concept Videos
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
02:54Canonical Wnt Signaling Pathway
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