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Updated: May 17, 2026
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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
Published on: June 27, 2017
Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in
F Heath Damron1, Elizabeth S McKenney, Herbert P Schweizer
1Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA.
Applied and Environmental Microbiology
|November 6, 2012
Abstract:
We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.
