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CARD-FISH for environmental microorganisms: technical advancement and future applications
1Department of Civil and Environmental Engineering, Tohoku University, Miyagi, Japan. kengok@epl1.civil.tohoku.ac.jp
Microbes and Environments
|November 6, 2012
Summary
Fluorescence in situ hybridization (FISH) with catalyzed reporter deposition (CARD-FISH) enhances environmental microorganism detection. This review highlights CARD-FISH advancements and applications in microbiology.
Area of Science:
- Environmental Microbiology
- Molecular Biology Techniques
Background:
- Fluorescence in situ hybridization (FISH) is a standard technique in environmental microbiology for detecting nucleic acids.
- FISH has been utilized for over two decades for the detection of ribosomal RNA, messenger RNA, and functional genes.
Purpose of the Study:
- To review the advancements and applications of Fluorescence in situ hybridization (FISH) combined with catalyzed reporter deposition (CARD).
- To focus on CARD-FISH technology for detecting environmental microorganisms.
Main Methods:
- Review of methodological improvements in CARD-FISH technology.
- Exploration of CARD-FISH in combination with other techniques and instruments.
Main Results:
- Significant methodological improvements have been made in CARD-FISH technology.
- CARD-FISH has expanded applications in environmental microorganism detection.
Conclusions:
- CARD-FISH represents a significant advancement over traditional FISH for environmental microbiology.
- Further integration of CARD-FISH with other methods promises enhanced microbial detection capabilities.
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