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Updated: May 17, 2026
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Detection of Extravascular Trypanosoma Parasites by Fine Needle Aspiration
Published on: August 7, 2019
[Fine needle aspirate of lymph node as the analytical sample for immunophenotyping]
Viktorija Svencbir1, Veronika Anić, Zoran Siftar
1Merkur University Hospital, Department of Clinical Cytology and Cytogenetics, Zagreb, Hrvatska. vsvencbir@yahoo.com
Summary
Fine needle aspiration (FNA) provides adequate material for flow cytometry, crucial for diagnosing lymphoproliferative diseases. This method accurately distinguishes benign from malignant lymph node lesions by determining B-lymphocyte clonality.
Area of Science:
- Hematology
- Cytopathology
- Immunology
Context:
- Flow cytometry is essential for diagnosing lymphoproliferative diseases by assessing B-lymphocyte clonality.
- Fine needle aspiration (FNA) is a common diagnostic procedure for lymph node evaluation.
Purpose:
- To evaluate the diagnostic value and sample adequacy of FNA for flow cytometry in lymph node lesions.
- To determine B-lymphocyte clonality in benign and malignant lymph node conditions.
- To assess the utility of FNA combined with flow cytometry for diagnosing lymphoproliferative diseases.
Summary:
- This study analyzed 239 patients, classifying them into B-cell non-Hodgkin's lymphoma, benign lymphoproliferative disease, and other categories.
- Results confirmed FNA yields sufficient cells for flow cytometry, with no inadequate samples.
- Flow cytometry is critical for differentiating benign from malignant conditions, especially in cases with monomorphic lymphoid cell populations.
Impact:
- FNA coupled with flow cytometry is a simple, safe, and effective method for diagnosing lymphoproliferative disorders.
- This approach aids in accurate diagnosis and separation of benign versus malignant lymph node pathologies.
- The findings support the routine use of FNA and flow cytometry in clinical laboratory settings for lymph node analysis.
