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Related Experiment Video

Updated: May 17, 2026

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Practical axial optical trapping.

A H Mack1, D J Schlingman, L Regan

  • 1Integrated Graduate Program in Physical and Engineering Biology, Yale University, New Haven, Connecticut 06511, USA.

The Review of Scientific Instruments
|November 7, 2012
PubMed
Summary
This summary is machine-generated.

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See all related articles

We developed a new calibration method for optical trapping to accurately measure molecular tension and extension. This technique improves DNA force measurements and enables force-clamp experiments for studying molecular dynamics.

Area of Science:

  • Biophysics
  • Optical Tweezers
  • Molecular Mechanics

Background:

  • Optical trapping is a powerful technique for measuring forces at the single-molecule level.
  • Accurate calibration is crucial for reliable force measurements in optical trapping experiments.

Purpose of the Study:

  • To present a novel, generally-applicable method for calibrating optical trapping measurements.
  • To accurately convert scattering intensity and stage displacement to applied tension and bead-coverslip separation.

Main Methods:

  • Developed a calibration procedure using light scattered from an untethered, trapped bead.
  • Accounted for optical aberrations and interference effects between scattered and trapping beams.
  • Applied the method to measure DNA force-versus-extension relationships.

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Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

Related Experiment Videos

Last Updated: May 17, 2026

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

Main Results:

  • Demonstrated accurate calibration by achieving expected extensible wormlike-chain (WLC) behavior for DNA.
  • Showcased the method's versatility with measurements across a range of laser intensities.
  • Successfully implemented a force-clamp functionality for dynamic molecular studies.

Conclusions:

  • The new calibration method enhances the accuracy and reliability of optical trapping measurements.
  • This technique provides a robust tool for investigating molecular mechanics and dynamics.
  • The force-clamp capability opens new avenues for studying real-time molecular events.