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Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
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Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Published on: May 4, 2020

Highly sensitive measurement of whole blood chromium by inductively coupled plasma mass spectrometry

Wendy Cieslak1, Kathleen Pap, Dustin R Bunch

  • 1Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA.

Clinical Biochemistry
|November 7, 2012
PubMed

Insights

This study developed a highly sensitive method for measuring chromium (Cr) in blood using ICP-MS. The new method establishes a reference interval of 3.92 to 7.48 nmol/L for total chromium in healthy adults.

Area of Science:

  • Clinical Chemistry
  • Trace Element Analysis
  • Mass Spectrometry

Background:

  • Chromium (Cr) is a vital trace element linked to diabetes and cardiovascular health.
  • Accurate blood chromium measurement is crucial for assessing nutritional status but is challenging due to matrix interferences and contamination.
  • A hypochromic state correlates with poor blood glucose control and lipid metabolism.

Purpose of the Study:

  • To develop a highly sensitive method for quantifying total chromium in whole blood using ICP-MS.
  • To validate a reference interval for blood chromium in a healthy local population.

Main Methods:

  • Inductively coupled plasma mass spectrometry (ICP-MS) with a collision/reaction cell was employed.
  • Interference was minimized using kinetic energy discrimination and helium as a selective collision gas.
  • Reference interval was determined in 51 healthy adults (19-64 years) using whole blood samples.

Main Results:

  • The developed assay demonstrated excellent linearity (3.42–1446.59 nmol/L) and accuracy (87.7–99.8%).
  • High sensitivity was achieved by effectively minimizing interference.
  • The validated reference interval for total chromium in whole blood was determined to be 3.92–7.48 nmol/L.

Conclusions:

  • The validated ICP-MS methodology offers high sensitivity and selectivity for total chromium measurement in whole blood.
  • This method provides a reliable tool for assessing chromium status.
Abstract

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