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Updated: May 17, 2026
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
[Construction of a recombinant stable Ba/F3 cell strain containing Tpr-Met]
Ling-fei Shu1, Wei Li, Yi-qun Zhan
1Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, China.
Aim:
To construct a stable cell strain encoding tumor-associated fused gene which expresses oncoprotein Tpr-Met.
Methods:
We transfected Tpr-Met vector into Ba/F3 cells and screened the cell strain stably expressing Tpr-Met. The interleukin 3 (IL-3) independent proliferation of the cells was measured using the MTS assay. The expression of Tpr-Met, the activity of downstream signal transduction pathway and SU11274-induced inhibition of the signal pathway were investigated by Western blotting.
Results:
We obtained a Ba/F3 cell strain stably expressing Tpr-Met. The cells presented IL-3 independent proliferation, suggesting a malignant transformation of the cell line. In Tpr-Met transformed Ba/F3 cells, the phosphorylation of Met and ERK were enhanced; however, specific c-Met inhibitor SU11274 suppressed the cell proliferation and c-Met phosphorylation.
Conclusion:
Tpr-Met transformed Ba/F3 strain has been successfully constructed.
