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Updated: May 17, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
High glucose selectivity in pressurized water hydrolysis of cellulose using ultra-fast reactors
Danilo A Cantero1, M Dolores Bermejo, M José Cocero
1Department of Chemical Engineering and Environmental Technology, University of Valladolid, Prado de la Magdalena s/n, 47011 Valladolid, Spain. da.cantero@iq.uva.es
Abstract:
A new reactor was developed for the selective hydrolysis of cellulose. In this study, the glucose selectivity obtained from cellulose was improved by using ultra-fast reactions in which a selective medium was combined with an effective residence time control. A selective production of glucose, fructose and cellobiose (50%) or total mono-oligo saccharides (>96%) was obtained from the cellulose in a reaction time of 0.03 s. Total cellulose conversion was achieved with a 5-hydroxymethylfural concentration lower than 5 ppm in a novel micro-reactor. Reducing the residence time from minutes to milliseconds opens the possibility of moving from the conventional m(3) to cm(3) reactor volumes.

