Mesenspheres of neural crest-derived cells enriched from bone marrow stromal cell subpopulation

Neural crest-derived cells (NCCs) can be used for cell replacement therapy of neurodegenerative diseases and nerve injury, and it is of significance to open readily accessible tissue sources for NCCs due to their insufficient supply. In this study, we aimed to examine the possibility of enriching NCCs from bone marrow stromal cell (BMSC) subpopulation. The epidermal growth factor/fibroblast growth factor-2 (EGF/FGF2)-responsive BMSC subpopulation (BMSC-C2) was isolated from rat bone marrow by repetitive two-step condition culture. The BMSC-C2 subpopulation showed a long-term proliferative capacity and high cell growth rate, and possessed a significant sphere-forming ability. The mesenspheres derived from BMSC-C2 subpopulation were self-renewable and could express NCC markers, such as CD29, CD44, nestin, CD133 and p75(NTR). In particular, the mesenspheres could be induced to differentiate into neuron- and glia-like cells in vitro. Collectively, our results might provide a basis for in-depth studies of recruiting postmigratory NCCs from bone marrow and various neural crest-derived tissues.