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Related Concept Videos

Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
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iPS Cell Differentiation

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Cellular Differentiation00:57

Cellular Differentiation

How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
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Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

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Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
10:07

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Published on: May 26, 2017

Skeletal muscle pericyte subtypes differ in their differentiation potential.

Alexander Birbrair1, Tan Zhang, Zhong-Min Wang

  • 1Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, North Carolina, Medical Center Boulevard, Winston Salem, NC 27157, USA.

Stem Cell Research
|November 7, 2012
PubMed
Summary

Skeletal muscle pericytes can generate neural progenitor cells for central nervous system therapies. A specific surface marker was identified for isolating these neural progenitor cells from non-transgenic species.

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Last Updated: May 17, 2026

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
10:07

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Published on: May 26, 2017

Isolation and Purification of Murine Cardiac Pericytes
10:49

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Published on: August 16, 2019

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
10:52

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Published on: August 21, 2014

Area of Science:

  • Cell Biology
  • Neuroscience
  • Regenerative Medicine

Background:

  • Neural progenitor cells are promising for CNS disorders but difficult to access.
  • Skeletal muscle-derived Tuj1+ cells' neural potential and origin remain unknown.
  • Identifying neural progenitor cell sources is crucial for cell-based therapies.

Purpose of the Study:

  • To identify the origin and potential of neural progenitor cells derived from skeletal muscle.
  • To characterize different pericyte subtypes in skeletal muscle.
  • To find a method for isolating neural progenitor cells for clinical applications.

Main Methods:

  • Utilized a double transgenic Nestin-GFP/NG2-DsRed mouse model.
  • Isolated and cultured two pericyte subtypes (type-1 and type-2).
  • Analyzed cell differentiation potential and surface marker expression.

Main Results:

  • Identified two pericyte subtypes: type-1 and type-2.
  • Demonstrated that type-2 pericytes generate Tuj1+ neural progenitor cells.
  • Type-2 pericyte-derived Tuj1+ cells retain some pericytic markers and express a unique surface marker (nerve growth factor receptor).

Conclusions:

  • Type-2 pericytes in skeletal muscle are bipotential, generating neural progenitor cells and pericytes.
  • Neural progenitor cells derived from type-2 pericytes can be identified and isolated using the nerve growth factor receptor.
  • This discovery offers a potential new source for neural progenitor cell therapy.